Abstract
Effect of norepinephrine on the phosphorylation of nuclear proteins was examined in cultured rat cardiocytes. Differential effects of phorbol esters, endothelin and platelet activating factor on the phosphorylation of a nuclear acid soluble fraction were evaluated. A specific phosphorylated nuclear acid soluble protein was identified in response to norepinephrine. The increase in phosphorylation in the norepinephrine-treated cardiocytes is significantly greater than 8 fold (p <0.001) as compared to the control group. This nuclear acid soluble protein has a high content of serine (> 12%) as well as acidic amino acids (> 20%) which is distinctively different from the histones as analyzed by amino acid composition. The specific phosphorylation of this nuclear protein (Mr 31 Kda) can be induced by norepinephrine within 5 minutes, indicating of an early gene activation event which modulates nucleosomal structure by phosphorylation.
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