Phosphorus removal from municipal wastewater through a novel Trichosporon asahii BZ: Performance and mechanism

Abstract

A yeast BZ was screened from a laboratory-scale anaerobic/aerobic reactor and designated as Trichosporon asahii through 26S rDNA gene sequence analysis. The screened BZ abated over 70% of phosphorus in municipal sewage with 2–10 mg/L phosphorus in the appropriate conditions. The yeast BZ had strong adaptability to pH and the dissolved oxygen, but the cultivation temperature, carbon source, the ratio of C/P and the ratio of N/P had a critical influence on the phosphorus abatement performance of yeast BZ. The analysis of phosphorus concentration in the wastewater, cells, and extracellular polymeric substances (EPS) suggested that about 55%–66% of the removed phosphorus was in the yeast cells and 34%–45% in the EPS. The proposed probable metabolic mechanism of phosphorus in yeast BZ showed that EPS acted as a dynamic phosphorous transfer station, and most of phosphorus was transferred into yeast cells through EPS transfer station. These findings have crucial implications for the development of a promising stable and easy-operation biological phosphorus abatement process for municipal wastewater treatment.