Abstract
SUMMARY: A procedure is described for the determinati on of phospholipid, ‘phosphoprotein’, ‘acid-soluble’ phosphate, pentosenucleic acid (DNA), deoxypentose-nucleic acid (DNA), and ‘insoluble’ polyphosphate in samples of mycobacteria of about 30 mg. dry weight. The content of each of these fractions in a number of cultures of Mycobacterium smegmatis, M. phlei and M. tuberculosis was determined. Considerable variation in some of the fractions was found in different cultures of the one organism, especially in the case of M. smegmatis. The effect of the age of a culture on its content of each fraction was studied for M. smegmatis. Studies were made on the changes in the phosphate fractions during the first few hours after the introduction of a heavy inoculum into a fresh medium.
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