Phosphorus Application Affects the Soybean Root Response to Water Deficit at the Initial Flowering and Full Pod Stages

Abstract

Application of phosphorus (P) fertilizer is an important factor for improving the tolerance to water deficit in many plants. A pot experiment was conducted to identify the effects of P application on soybean adaptability to water deficit at the R1 (initial flowering) and R4 (full pod) stages through the investigation of root morphological traits, plant P uptake and resultant yield in two soybean (Glycine max L. Merrill) cultivars (Dongnong 46 and Heisheng 101). The four levels of P application were 0, 7.3, 14.6 and 29.2 mg kg−2, respectively. The three water treatments were (1) 65–75% of field water capacity (FWC) as a well-watered control, (2) 30–40% of FWC at the R1 stage, and (3) 30–40% of FWC at the R4 stage. Root traits, plant uptake of P and yield were significantly reduced by water deficiency at different growth stages, especially at the R4 stage. Application of P enabled to alleviate the adverse effects of water deficit, to increase the root dry weight, root length and root surface area, and to slow root senescence after the R5 (initial pod filling) stage. The response of soybean genotypes to both water and P deficit was different. In the absence of P application, Dongnong 46 showed relatively low adaptability to water deficit at the R4 stage, whereas Heisheng 101 showed a lower reduction of root traits and yield. The beneficial effects of P application for Dongnong 46 were more pronounced than those for Heisheng 101. Based on this experiment, we suggested that P fertilizer application to soybean may be justified in low-rainfall years because of its ability to enhance the soybean adaptability to water deficit stress by improving the root morphology, P uptake and consequently yield.