Abstract
Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. However, studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development.
Highlights
Phosphorylation is one of the most important post-translational modifications (PTMs) of proteins (Pawson and Scott, 1997)
The enrichment methods of phosphoproteins/phosphopeptides from complex mixtures have greatly improved over the years
Combining the titanium (Ti4+)-based Immobilized metal affinity chromatography (IMAC) and the reverse phase (RP)strong cation exchange (RP-SCX) biphasic trap column-based online RPLC is a great example of the advancements (Bian et al, 2012; Wang et al, 2013b)
Summary
Jinna Li 1, Cecilia Silva-Sanchez 2, Tong Zhang 3, Sixue Chen 1, 2, 3 and Haiying Li 1*. Reviewed by: Christof Rampitsch, Agriculture and Agrifood Canada, Canada Mohammad-Zaman Nouri, Rice Research Institute of Iran in Mazandaran, Iran. Protein phosphorylation has long been recognized as an essential mechanism to regulate many important processes of plant life. Studies on phosphorylation mediated signaling events in plants are challenged with low stoichiometry and dynamic nature of phosphorylated proteins. Significant advances in mass spectrometry based phosphoproteomics have taken place in recent decade, including phosphoprotein/phosphopeptide enrichment, detection and quantification, and phosphorylation site localization. This review describes a variety of separation and enrichment methods for phosphoproteins and phosphopeptides, the applications of technological innovations in plant phosphoproteomics, and highlights significant achievement of phosphoproteomics in the areas of plant signal transduction, growth and development
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