Abstract
Verticillium wilt is a plant vascular disease causing severe yield and quality losses in many crops and is caused by the soil-borne plant pathogenic fungus Verticillium dahliae. To investigate the molecular mechanisms of the cotton–V. dahliae interaction, a time-course phosphoproteomic analysis of roots of susceptible and resistant cotton lines in response to V. dahliae was performed. In total, 1716 unique phosphoproteins were identified in the susceptible (S) and resistant (R) cotton lines. Of these, 359 phosphoproteins were significantly different in R1 (1 day after V. dahliae inoculation) vs R0 (mock) group and 287 phosphoproteins in R2 (3 days after V. dahliae inoculation) vs R0 group. Moreover, 263 proteins of V. dahliae-regulated phosphoproteins were significantly changed in S1 (1 day after V. dahliae inoculation) vs S0 (mock) group and 197 proteins in S2 (3 days after V. dahliae inoculation) vs S0 group. Thirty phosphoproteins were significantly changed and common to the resistant and susceptible cotton lines following inoculation with V. dahliae. Specifically, 92 phosphoproteins were shared in both in R1 vs R0 and R2 vs R0 but not in susceptible cotton lines. There were 38 common phosphoproteins shared in both S1 vs S0 and S2 vs S0 but not in resistant cotton lines. GO terms and Kyoto Encyclopedia of Genes and Genomes pathway analyses displayed an abundance of known and novel phosphoproteins related to plant–pathogen interactions, signal transduction, and metabolic processes, which were correlated with resistance against fungal infection. These data provide new perspectives and inspiration for understanding molecular defense mechanisms of cotton roots against V. dahliae infection.
Highlights
Cotton (Gossypium spp.) is one of the most important economic crops in the world and is the main source of natural textile fibers
It has been found that the tolerant and resistant plant genotypes are less sensitive to V. dahliae and activate defense responses to V. dahliae.[5−7] a number of studies report on plant−V. dahliae interactions, there remains limited knowledge about the molecular mechanism of plants against V. dahliae.[5,8,9]
This study provides valuable data and novel insights for further investigations into molecular events involved in cotton defense responses to V. dahliae
Summary
Cotton (Gossypium spp.) is one of the most important economic crops in the world and is the main source of natural textile fibers. Causes plant vascular diseases[1] and often results in severe cotton yield reductions and losses.[2,3] The pathogen can adhere to the root surface, and its hyphae penetrate the roots and colonize the cortex They extend into the xylem and form conidia.[4] the toxic, cell-wall-degrading enzymes and elicitor-like substances are produced by V. dahliae to suppress plant innate immune responses.[3] It has been found that the tolerant and resistant plant genotypes are less sensitive to V. dahliae and activate defense responses to V. dahliae.[5−7] a number of studies report on plant−V. dahliae interactions, there remains limited knowledge about the molecular mechanism of plants against V. dahliae.[5,8,9]. This study provides valuable data and novel insights for further investigations into molecular events involved in cotton defense responses to V. dahliae
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