Abstract
As an alpine plant, Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress. We adopt the phosphoproteomic and proteomic analysis combining with physiological measurement to illustrate the responsive mechanism of R. chrysanthum seedling under cold (4°C) stress. After chilling for 12h, 350 significantly changed proteins and 274 significantly changed phosphoproteins were detected. Clusters of Orthologous Groups (COG) analysis showed that significantly changed phosphoproteins and proteins indicated cold changed energy production and conversion and signal transduction. The results indicated photosynthesis was inhibited under cold stress, but cold induced calcium-mediated signaling,reactive oxygen species (ROS) homeostasis and other transcription regulation factors could protect plants from the destruction caused by cold stress. These data provide the insight to the cold stress response and defense mechanisms of R. chrysanthum leaves at the phosphoproteome level.
Highlights
As an alpine plants,Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress
In order to reduce limit cold-induced damage, plants especially alpine plants have evolved multiple stress-tolerant strategies, which contribute to protect plants from destruction caused by cold stress [4]
Photosynthesis of rhododendron chrysanthum is very sensitive to cold stress
Summary
As an alpine plants,Rhododendron chrysanthum (R. chrysanthum) has evolved cold resistance mechanisms and become a valuable plant resource with the responsive mechanism of cold stress. Cold stress directly leads to membrane rigidification and protein denaturation, and indirectly reduces the activities of enzymes [2], inhibition of protein synthesis and degradation, as well as considerable membrane damage [3]. In order to reduce limit cold-induced damage, plants especially alpine plants have evolved multiple stress-tolerant strategies, which contribute to protect plants from destruction caused by cold stress [4]. Photosynthetic process of plants is inevitably changed in response to cold stress, especially PSII. The PSII photoinhibition appeared in order to protect PSI at cold stress [5]. Cold stress induced the changes of a variety of protein kinases and transcription factors in plants [6]. Various abiotic stresses result in excessive accumulation of reactive oxygen species (ROS) in plants, and the effective antioxidant enzymes such as catalase (CAT), Superoxide dismutase (SOD) and peroxidase (POD) could eliminate excessive ROS [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
