Abstract
Phosphoproteomics-based peptide ligand-receptor kinase pairing. Commentary on: "A peptide hormone and its receptor protein kinase regulate plant cell expansion".
Highlights
Small signaling peptides and their receptors play essential roles in plant growth and development (Murphy et al, 2012; Czyzewicz et al, 2013; Matsubayashi, 2014)
Phosphoproteomics-based peptide ligand-receptor kinase pairing such studies and there is the requirement for expressing the receptor in N. benthamiana
Overexpression of individual A. thaliana leucine-rich repeat receptor kinases (LRR-RKs) from subfamilies X and XI in tobacco BY-2 cells was combined with photoaffinity labeling of these LRR-RKs by a biologically active small signaling peptide analog derivatized with 125I-labeled photoreactive 4-azidosalicylic acid ([125]ASA)
Summary
Small signaling peptides and their receptors play essential roles in plant growth and development (Murphy et al, 2012; Czyzewicz et al, 2013; Matsubayashi, 2014). A peptide hormone and its receptor protein kinase regulate plant cell expansion by Haruta, M., Sabat, G., Stecker, K., Minkoff, B. Arabidopsis thaliana encodes over 600 putative receptor-like kinases and more than 1000 potential secreted peptides (Shiu and Bleecker, 2001; Lease and Walker, 2006), and similar numbers can be expected in other plant species (Shiu et al, 2004; Lehti-Shiu et al, 2009).
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