Phospholipids and cholesterol of liver nuclei during artificial hypobiosis of rats

Abstract

The contents of lipids in the tissue and the nuclei of liver cells during artificial hypobiosis, as well as in the nuclei of liver cells for 3 days after the cessation of cooling in rats, were studied. In the artificial hypobiosis and in the state of normothermia 24 h after the cessation of cooling, the total phospholipid content of the liver cell nuclei increased by 20% due to minor phospholipids. The levels of sphingomyelin, phosphatidylinositol, phosphatidylserine, cardiolipin, and lysophosphatidylcholine were doubled in hypobiosis and then nonmonotonically returned to the normal level within 72 h. In the state of artificial hypobiosis, the levels of fatty acids, cholesterol, and diglycerides increased by 30–40%. The state of artificial hypobiosis did not affect the level of lipids in the liver tissue of Wistar rats. The increase of the lipid content in the liver cell nuclei of Wistar rats indicates the important role of lipids in functions of the nucleus when the energy supply and protein synthesis are inhibited under conditions of artificial hypobiosis.