Abstract
Uterine myoma is a common gynecological tumor and affect nearly one fourth of middle –age wome. It is believed that estrogen and progesterone are important for the proliferation /differentiation of both endometrium and myometrium –the tissue that uterine myoma arising from. However, little is known about the molecular mechanism regulate the tumorigenesis of uterine myoma. We have identified genes expressed differentially between proliferative and secretory endometrium by differential display RTPCR (DDRTPCR), and we tried to explore the role of one of these genes , phospholipid scramblase 4 (PLSCR 4) in the endometrium and uterine myoma. We engineered the DNA construct of PLSC 4 fragment and expressed in E. coli. The fusion protein was purified and used to immunize the rabbit. The specificity and sensitivity of the resultant rabbit anti-PLSCR4 serum were confirmed . Immunhistochemistry assay of endometrium using this anti-PLSCR 4 serum were performed but no significant difference between prliferative and secretory endometrium was noted. The uterine myoma tissues were also analyzed by immunochemistry and western blot, and it showed the expression of PLSCR 4 are lower in uterine myomas than the normal myometrium. This finding suggest the PLSC 4 may play roles in cell apoptosis as other members in the phospholipid scramblase family, and the lower than normal expression may be involved in the tumorigenesis.
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