Abstract
Abstract—Neuronal and glial cells were isolated from the brains of 17‐day old rats and incubated for 5 h with either radioactive inorganic phosphate, palmitate, serine, choline or ethanolamine in a tissue culture medium. A comparison of the results suggests that both neuronal and glial cells exhibit effective de novo, phospholipid synthesis and that the observed differences in the uptake are due more to quantitative rather than qualitative differences in phospholipid metabolism of both cell types. Incubations of the combined neuronal and glial fractions with 32PO4 and [3H]palmitate result in incorporations up to 100% higher than calculated from incubations of the separate fractions, suggesting that phospholipid metabolism of neuronal and glial cells may exhibit cooperativity.
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