Abstract
1. The effects of lipid-protein interactions on carp brain and liver mitochondrial MAO with respect to substrate and inhibitor preference, thermostability and Arrhenius parameters were studied and compared. 2. Treatment with phospholipase A 2, C or D decreased MAO activities towards 5-hydroxytryptamine (5-HT), β-phenylethylamine and tyramine similarly, accompanied by great changes in their apparent affinities for MAO, but not by changes in V max values. 3. Minimum phospholipid binding to mitochondria might be essential for enzyme activity. 4. Among these activities, 5-HT deamination was the most sensitive to the changes in mitochondrial phospholipids and bulk lipid phase transition (fluidity). 5. Sensitivity of MAO to clorgyline or l-deprenyl was not affected by these phospholipase treatments. 6. Of the phospholipids tested, only phosphatidylinositol significantly activated MAO activity towards 5-HT in both intact and phospholipase-treated mitochondria.
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