Abstract
Abstract Established microbial ecology analytical techniques for measuring the quantity and activity of bacteria were examined for use on biological granular activated carbon (GAC). Activity was determined using the fluorescein diacetate (FDA) assay. The assay was tested and accordingly corrected to account for the adsorption of the fluorescein on the GAC by utilizing a standard curve containing GAC from the reactor. The quantity of FDA solution and incubation times were also examined. Normalization of fluorescein adsorbance to incubation time appears to be feasible provided that the concentration of the FDA solution does not become inhibiting. The amount of biomass present was determined by measuring the phosphorous content of the phospholipids in cellular membranes. The assay can be normalized for sample size. The combination of the two assays successfully measured the microbial characteristics of the biological GAC.
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Schedule a callMore From: Journal of Environmental Science and Health . Part A: Environmental Science and Engineering and Toxicology
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