Abstract
Phospholipase D-mediated transphosphatidylation to phosphatidylserine from phosphatidylcholine was successfully performed using subcritical 1,1,1, 2-tetrafluoroethane (R134a) as reaction medium. Phospholipase D from Stretomyces sp was found to be active in subcritical R134a at 30-50°C and 2-8MPa; Higher velocity and conversion were observed with respect to those attained in conventional organic solvents. A maximum conversion of 84.88% was attained at 6 MPa, 40°C, after 240 minutes with 9.6 units phospholipase D.
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