Abstract
21-day-old rat brain contains a soluble phospholipase C with the ability to hydrolyse phosphatidylcholine. This enzyme has an alkaline pH optima. The results of the DEAE-cellulose fractionation, the pH profile and the Ca 2+-dependency suggest that the enzyme may be the same as that responsible for phosphatidylinositol hydrolysis. The activity of the phospholipase C is associated closely with a diacylglycerol lipase. The two enzyme activities could be separated by DEAE-cellulose fractionation, resulting in greater than 100% apparent recovery of the phospholipase C activity. This phospholipase C activity is not the result of the back reaction of choline phosphotransferase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
