Phospholipase A 2 inhibits nuclear nucleoside triphosphatase activity and mRNA export in isolated nuclei from rat liver

Abstract

The present study is undertaken to investigate whether the phospholipase A 2 (PLA 2) influences mRNA nucleocytoplasmic transport evaluated by nucleoside triphosphatase (NTPase) activity and mRNA export in isolated hepatic nuclear envelope. Isolated hepatic nuclei from rat liver were exposed to PLA 2 (10 −5 ∼ 10 −2/ml) with or without incorporation of nuclei with phosphatidylcholine (PC) liposome. Messenger RNA exports and NTPase activities of nuclear membrane were assayed using ATP and GTP as substrates. We found that the RNA efflux, evaluated by [ 3H] uridine, was potently decreased in a concentration-dependent manner, by incubation of hepatic nuclei with PLA 2, regardless using ATP or GTP as substrates. The PC content in nuclear membrane was also decreased by PLA 2-treatment. The PC was incorporated into the nuclear membrane by addition of phospholipid liposomes into the incubation mixture. PC incorporation into the nuclear membrane did not alter mRNA export. However this resulted in a significant increase in mRNA export rate in PLA 2-treated group. Messenger RNA export rate in PLA 2 (10 −3 unit/mL)- treated nuclear membrane was positively correlated with level of PC incorporation, both using ATP and GTP as substrates. The activity of nucleoside triphosphatase, a nuclear membrane-associated enzyme, showed parallel variations with mRNA transport. It is concluded that nuclear PLA 2 plays a regulatory role in RNA transport, which can be antagonized by exogenous PC. These might be pathophysiologically significance, although the mechanisms by which this effect takes place remain to be clarified.