Abstract
Protein activator of cyclic nucleotide phosphodiesterase from bovine brain (9 μg/0.5 ml) was found to stimulate ATP-dependent Ca 2+ - transport in dog cardiac microsomal preparations enriched in sarcoplasmic reticulum. Both oxalate-dependent calcium uptake and ATP-dependent calcium binding were increased. Cyclic AMP-dependent protein kinase (Sigma, type 1, 25 μg/0.5 ml) and cyclic AMP (1 μM) also stimulated calcium uptake and the presence of a maximal stimulatory concentration of the phosphodiesterase activator produced an additive elevation of calcium uptake indicating separate mechanisms of action and potentially different modulatory roles for these two systems in the control of calcium transport.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.