Abstract
The phosphatide components of fowl blood cells were separated by chromatography on silicic acid-impregnated paper and found to consist of lecithin (42%), phosphatidyl ethanolamine (24%), sphingomyelin + lysophosphatidyl ethanolamine (21%), and small amounts of phosphatidyl serine, lysolecithin, phosphatidyl inositol and phosphatidic acid. Each component had a different fatty acid composition; in particular, phosphatidyl ethanolamine had a higher proportion of polyunsaturated fatty acids and of stearic acid, and a lower proportion of palmitic acid than lecithin. These two phosphatides were also present in their respective plasmogen forms, the aldehyde moieties of which were found to be mainly palmitaldehyde and stearaldehyde. Incubation of the total blood cells with [2− 14]acetate resulted in the incorporateion of 14C into the non-phosphatide fraction (cholesteryl esters, cholesterol and diglycerides) and to a lesser extent into the lecithin, phosphatidyl ethanolamine and sphingomyelin components.
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