Phosphate ions detection by using an electrochemical sensor based on laser-scribed graphene oxide on paper

Abstract

In this work, electrodes based on laser-scribed reduced graphene oxide were fabricated using filter paper as the substrate. To fabricate the electrodes, a water suspension of graphene oxide was filtered to produce a continuous and uniform film of graphene oxide on the filter paper surface. Subsequently, a CO2 laser was used to “write” the working, counter and reference eelctroes by reducing graphene oxide in specific areas to define complete sensors. Referecnce electrodes were then coated with a commercial Ag/AgCl conductive paste to produce a quasi Ag/AgCl reference. As fabricated devices were employed as electrochemical sensors for detection of phosphate ions in water by employing the molybdenum blue method. This method exploits the reaction between molybdate and phosphate ions in acidic media leading to a Keggin-type complex (PMo12O403−) which, being electrochemically active, enables the indiret detection of phosphate ions. Sensors exhibited high selectivity and sensitive detection of phosphate ions in a wide linear range, from 1 to 20 µM; with a limit of detection of 0.4 µM. To demonstrate that sensors could be utilized for in-situ phosphate ion detection, paper substrate was first pre-loaded with sulphuric acid and molybdate ions. During analysis, these chemicals were then desorbed directly into the test sample eliminating the need for any kind of external manipulation or reagent addition. Thus, this paper presents the fabrication of a portable, easy-to-use, biodegradable and fast phosphate ions sensor for in situ and real-time monitoring of water quality.