Abstract
The absolute specificity for ADP as phosphate acceptor during oxidative phosphorylation is lost upon sonic treatment of mitochondria. 1. 1. The specificity varies with the substrate and thus with the number of phosphorylation sites involved. 2. 2. K m values for IDP and ADP as phosphate acceptors with various substrates and at optimal pH have been determined. 3. 3. Uncouplers, monovalent ions and oligomycin all affect the phosphorylation of IDP more strongly than that of ADP. 4. 4. The nonspecific reaction is not due to the involvement of a nucleoside diphosphate kinase reaction. The ability to phosphorylate the various nucleoside diphosphates is considered to be dependent on the affinity between these nucleotides and the phosphorylating enzyme and may reflect the endogenous condition when the translocase has lost its physiological rôle.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
