Abstract

Western blot analysis indicated that WB cells contained multiple proteins of molecular mass 42–47 K which reacted with anti-connexin 43 antibody. Incubation of cells with 12- O-tetradecanoylphorbol-13-acetate (TPA) for 5 min caused the inhibition of dye transfer and the appearance of reactive material of 50 K which was abolished by acid phosphatase. Longer term incubation with TPA caused loss of connexin 43 protein.

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