Phân lập vi rút SENPV từ sâu xanh da láng (Spodoptera exigua Hüber) tại Đồng bằng sông Cửu Long

Abstract

The experiment was aimed to identify species of the entomopathogenic virus by using a traditional method (observing symptoms and describing characteristics) and the molecular method based on PCR amplification with specific primers PSF002 and PER001 for nucleopolyhedrovirus (NPV). There were 29 samples from the diseased larva of the beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae) collected in the Mekong Delta. The results from observation and description of the symptoms, as well as the extraction of DNA and PCR amplification with specific primers PER001 and PSF002 indicated that 20 samples had unique fragments approximated 550 bp which was identified nucleopolyhedrovirus belonged to Baculoviridae family. They include seven strains collected in Vinh Long province, five strains in Dong Thap province, three strains in An Giang province and five strains collected in Can Tho city.