Phân lập và tuyển chọn một số dòng nấm từ gỗ mục có khả năng loại màu thuốc nhuộm ở Đồng bằng sông Cửu Long

Abstract

The main objective of this study was to isolate and identify indigenous white rot fungi for bioremediation of dye textile compounds. Fungal fruit body samples were collected from decaying woods in the Mekong Delta for isolation. The dye textile compound decolourisation capacity of isolated fungi was tested on MT3 (Jonathan and Fasidi, 2001) containing 500 mgL-1Brilliant Black BN or Bromophenol blue. The results showed that 54 fungal isolates were isolated from decaying wood. Twelve out of fifty four fungal isolates showed their capacity in decolourisation of Bromophenol blue. The HG1 strain was able to degrade 493 mg.L-1 Bromophenol blue, corresponding to 92% within 8 days of incubation, while fifteen out of fifty four fungal isolates showed their capacity in decolourisation of 1Brilliant Black BN. The maximum decolourisation of this compound was 99% (493 mg.L-1) within 7 days of incubation by TV13 strain . HG1 and TV13 were identified as the best candidates for decolourisation of Brilliant Black BN and Bromophenol blue compounds, respectively. Based on the results of 18S-rRNA sequences, these two candidates were genetically and relatively identified as genus of Marasmiellus. Thus, these two fungal isolates were relatively identified as Marasmiellus sp. HG1 and Marasmiellus sp. TV13.