Phân lập, nhận diện vi khuẩn phân hủy tinh bột từ rác hữu cơ, ruột sùng (Holotrichia parallela) và trùn đất (Lubricus terrestris)

Abstract

This research was carried out with the purpose of isolation and selection of promising indigenous starch-degrading bacteria in order to treat starch waste from starch processing factories. For that objective, the traditional techniques and modern molecular techniques were used for the isolation and identification of starch degrading bacterial strains from the organic wastes landfills, guts of Holotrichia parallela and Lubricus terrestris. The study’s result performed that there were 58 bacterial strains isolated on 1% starch agar. Most of the them were rod-shaped, motile, Gram positive, endo-spore forming, positive in catalase as well as Methyl red test. Through the experiment evaluating the ability of starch degradation, 57/58 bacterial trains performed the secretions of amylase enzyme to break down starch in agar medium to form clear zones when dying with Lugol’solution. Of these, five promising strains RB8, RB17, SB25, TB6 and TB16 were selected for sequencing 16S rRNA gene and identified as Bacillus flexus, Bacillus subtilis, Bacillus megaterium, Bacillus cereus and Bacillus flexus, respectively.