Abstract
In this work, 2-(2-aminoethoxy) ethanol-blocked phenylboronic acid-functionalized magnetic nanoparticles (blocked PMNPs) were fabricated for selective enrichment of different types of saccharides. The phenylboronic acid was designed for capturing the cis-diols moieties on saccharides molecules, and the 2-(2-aminoethoxy) ethanol can deplete the nonspecific absorption of peptides and proteins which always coexisted with saccharides. For mass spectrometry analysis, the PMNPs bound saccharides can be directly applied onto the MALDI plate with matrix without removing the PMNPs. By PMNPs, the simple saccharide (glucose) could be detected in pmol level. The complex saccharides can also be reliably purified and analyzed; 16 different N-glycans were successfully identified from ovalbumin, and the high-abundance N-glycans can be detected even when the ovalbumin was in very low concentration (2 μg). In human milk, ten different oligosaccharides were identified, and the lactose can still be detected when the human milk concentration was low to 0.01 μL.Graphical Electronic supplementary materialThe online version of this article (doi:10.1007/s41048-015-0002-3) contains supplementary material, which is available to authorized users.
Highlights
Glycosylation is one of the most common and important biological phenomena of post-translational modification processes in eukaryotic cells, playing a vital role in the modulation of protein functions (Wang et al 2006)
Vibrating sample magnetometer (VSM) spectra showed that the saturation magnetization of the nanoparticles decreased ; when it had been doped into silica nanoparticles and experienced further surface modification by APS and phenylboronic acid (PBA), the resulted PBA-functionalized magnetic nanoparticles (PMNPs) demonstrated superparamagnetic property (Fig. 1C (2–4)); after the modification, the saturation magnetization of the blocked PMNPs was 26.6 emu/g
We have explored a facile method for selective enrichment and analysis of saccharides
Summary
University, Baoding 071002, China 4 Fu Jen Catholic University, Taipei 24205, China. Received: 18 January 2015 / Accepted: 1 April 2015 / Published online: 14 July 2015
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