Abstract
1. 1. Phenylalanine hydroxylation has been demonstrated to occur in isolated rat kidney tubules under physiological conditions. 2. 2. The hydroxylation flux response is hyperbolic with apparent K m and V max values of ca 85μM phenylalanine and 49 nmol tyrosine formed/mg dry wt per hr respectively. 3. 3. Hydroxylation in kidney tubules is substantially less sensitive to effectors of cyclic AMP turnover and Ca + mobilization than phenylalanine hydroxylation in isolated liver cells.
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