Phenoxazinone synthase mimicking activity of a dinuclear copper(II) complex with a half salen type Schiff base ligand

Abstract

A dinuclear copper(II) complex [LCu(μ1,1-N3)2CuL] {where HL = (2-(3-aminopropylimino)methyl-6-methoxyphenol)} has been synthesized and characterized by several analytical techniques including single crystal X-ray diffraction analysis. Interesting supramolecular interactions are observed in the solid state of the complex. An unusual N–H⋯π interaction is observed where the chelate ring, Cu(1)–N(1)–Cu(1)b–N(1)b, [where b = 1 − x, −y, 1 − z] behaves as a π system. Supramolecular interactions have been related with Hirshfeld surface calculations. The complex has been evaluated as functional model for phenoxazinone synthase enzyme by using o-aminophenol (OAPH) as a model substrate in acetonitrile medium. Kinetics of the reaction was followed spectrophotometrically, which confirm that catalytic reactions follow Michaelis–Menten enzymatic reaction kinetics.