PHENOTYPING THE LEVEL OF BLOOD PRESSURE BY TELEMETRY IN MICE

Abstract

1. Using telemetry, arterial blood pressure (BP) can be measured directly over long periods in freely behaving animals without recent anaesthesia or surgery. In the present review, we discuss the strengths and limitations of this method and important considerations in using the method to characterize the BP level in mice. 2. A variety of informative statistics can be used to describe the BP level and we have made available a spreadsheet template for their calculation on a routine basis. The BP level is well summarized using the average value for an entire 24 h period or for the individual light and dark phases of the day. Such long-term averages exhibit less statistical variation than those of short recording periods. In addition, averages of the dark and light phases of the day convey information concerning circadian variations of BP. 3. The frequency distribution of BP samples provides additional information concerning the range of BP values recorded over the course of the day and can be described in terms of percentiles of the distribution that correspond with the minimum and maximum BP values and their span. 4. In mice, BP can be markedly affected by locomotor activity cycles that occur frequently throughout both the light and dark phases of the day. In addition, BP is strongly affected by ambient temperature and food intake, as well as potentially by other determinants of energy balance. Consideration of these factors may help improve accuracy and precision when phenotyping the BP level in mice.