Phenotypical and Molecular Characterization of Microsporum canis Strains in North-Tunisia

Abstract

In this study, 40 Microsporum canis isolates were obtained from different patients from the Mycology Unit of the Hospital La Rabta (Tunis) during a 3 month period. The phenotypic identification was done by morphological characterization and biochemical tests. Molecular analysis was performed by amplification of the ITS region of rDNA, the amplified region was subjected to enzymatic digestion and sequenced to evaluate phylogenetic relationships. The morphological analysis showed a considerable diversity of colonies as well as different morphologies of conidia and we have noted variability in the assimilation of the nitrogen and carbon sources. The PCR-RFLP results showed only one restriction pattern for each enzyme. The phylogenetic tree proves that all the strains from Tunisian patients are clonal and related with other strains from different origins. The classical methods used in the mycological laboratories are time-consuming, the PCR-RFLP analysis of the ITS is a reliable tool for the identification of M. canis strains. M. canis from infected Tunisian patients are clonal, although the isolates had different phenotypic characteristics.