Abstract
In this study, an Aeromonas hydrophila identified as a denitrifying bacterium by PCR detection of nitrate reductase (narG) and nitrite reductase (nirK) genes was incubated in seawater microcosms for 8 months at room temperature and at 4 °C. A study of the phenotypic variation demonstrated that A. hydrophila becomes gelatinase-positive after the incubation in sea water. We noted that starved A. hydrophila becomes unable to produce leucine arylamidase, and that the starved strain appears to grow more slowly. Indeed, we also observed a severe decrease in cellular aggregation of Aeromonas after incubation. In addition, atomic force micrographs revealed a reduction in cell size.
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