Abstract

In the present study, eight single-spore strains of Botrytis cinerea were isolated from tomato greenhouses located in Bejaia regions (Northern Algeria). Isolates were molecularly characterized by nine microsatellite markers. Isolates were assigned to B. cinerea and B. pseudocinerea with four isolates of each species. Morphological characterization was established using two cultures media Potato Dextrose Agar and Malt Extract Agar. All isolates inoculated on PDA medium were exclusively Sclerotial and Mycelial on MEA medium. Aggressiveness of both species was similar on tomato leaves and apple fruits. Moreover, B. cinerea isolates were more aggressive than B. pseudocinerea on lettuce leaves. Tomato and lettuce leaves were significantly more susceptible to the both fungi. A negative correlation was established between aggressiveness and morphological type. Phenotypic variability is considered of major importance to explain the epidemiology of the two cryptic species.

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