Abstract
Ecotropic murine leukemia viruses (MuLV) were capable of infecting mink, rabbit, duck, guinea pig, human, and cat cells, but not chicken cells, when phenotypically mixed with xenotropic MuLV. Mink cell cultures inoculated with the phenotypically mixed pools showed XC plaques with one-hit dose response kinetics; these plaques represented colonies derived from outgrowth of single infected cells rather than spreading areas of infection, which showed two-hit kinetics. Clonal lines of mink and rabbit cells chronically infected with N-tropic ecotropic (AKR-L1), B-tropic ecotropic (WN1802B), and NB-tropic ecotrpic (Friend) MuLV were established. Production of virus by these xenogenic cell lines was generally much lower than by mouse cells, as was the number of cells with gs-antigen detected by immunofluorescence. However, essentially all cells registered as virus-producers on infectious center plating. Treatment with IUdR enhanced virus production by 10-fold, and immunofluorescence staining as well, in the three infected mink cell lines. The viruses retained their ecotropic and Fv-1-determined host range characteristics through more than 6 to 12 months of chronic virus production in the heterologous cells.
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