Abstract
Endothelial cell (EC) proliferation is a crucial event in physiological and pathological angiogenesis. MicroRNAs (miRNAs) have emerged as important modulators of the angiogenic switch. Here we conducted high-content screening of a human miRNA mimic library to identify novel regulators of EC growth systematically. Several miRNAs were nominated that enhanced or inhibited EC growth. Of these, we focused on miR-26b, which is a conserved candidate and expressed in multiple human EC types. miR-26b overexpression enhanced EC proliferation, migration, and tube formation, while inhibition of miR-26b suppressed the proliferative and angiogenic capacity of ECs. A combinatory functional small interfering RNA (siRNA) screening of 48 predicted gene targets revealed that miR-26b enhanced EC growth and survival through inhibiting PTEN expression. Local administration of miR-26b mimics promoted the growth of new microvessels in the Matrigel plug model. In the mouse model of hindlimb ischemia, miR-26b was found to be downregulated in endothelium in the first week following ischemia, and local overexpression of miR-26b improved the survival of capillaries and muscle fibers in ischemic muscles. Our findings suggest that miR-26b enhances EC proliferation, survival, and angiogenesis. miR-26b is a potential target for developing novel pro-angiogenic therapeutics in ischemic disease.
Highlights
Blood vessels remain mostly quiescent throughout adult life
The screening results showed 129 miRNAs enhanced Endothelial cell (EC) growth by more than 0.85 log fold change when compared with the ECs treated with the miRNA controls, and 182 miRNAs reduced the EC growth (Figure 1A)
Based on data retrieved from small RNA sequencing datasets (Encyclopedia of DNA Elements [ENCODE]), we found 24 were both evolutionarily conserved and accounted for their expression in four different EC sources: human umbilical vein EC (HUVEC), human coronary artery endothelial cells (HCAECs), human aorta endothelial cells (HAECs), and human dermal micro-vascular endothelial cells (HMVECs) (Figures 1C and 1D)
Summary
Blood vessels remain mostly quiescent throughout adult life. In response to injury or pathological conditions, they maintain the capacity to rapidly form a new vascular network from pre-existing vessels in a complex process called angiogenesis.[1] During sprouting angiogenesis, while tip cells are highly migratory endothelial cells (ECs) that guide the new sprout toward pro-angiogenic gradients, neighboring stalk cells elongate the new sprout by their highly proliferative capacity.[2] EC proliferation is involved in other types of post-natal angiogenesis, such as enlargement of pre-existing capillaries and bridging or intussusception of enlarged vessels to form smaller daughter vessels.[3]. Thereby, the induction of angiogenesis in ischemic vascular disease would be beneficial, including enhancing EC growth, which is a hallmark of angiogenesis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
