Phenotypic heterogeneity of the progeny of streptomyces griseus conidia

Abstract

In order to understand the complex ontogenetical processes, the development of Streptomyces (S.) griseus was applied as a model. The developmental cycle of S. griseus starts and ends as a conidium. In between, coenocytic mycelium develops which, if studied by cytomorphological or biochemical methods, exhibits conspicious heterogeneity. The hyphae develop into young, transient and old vegetative hyphae and different stages of reproductive forms. In developmentally blocked mutants these sequences of events appear mixed in all possible associations. It seems as if the program of development could be divided into several subprograms. The quantitative evaluation of the results show that the individual morphological markers exhibit certain independence from each other realized with a given probability. The conidia of S. griseus are also heterogeneous concerning all morphological and physiological traits examined so far (shape, size, light refraction, staining and shape of nucleoids with Feulgen, methyl green--pyronine, intensity and form of polysaccharide distribution, heat resistance, etc.). Kinetics of the survival curves of two S. griseus strains--a well-sporulating and its developmentally blocked mutant /24/--are different from each other, one has many more heat resistant conidia than the other but the kinetics of the survival curves of the two S. griseus strains indicate that spore populations of both react differently to heat treatment and heat resistance can be modeled by assuming the presence of two independent subpopulations of spores with different heat sensitivity. The emergence of two distinct subpopulations with (possibly) the same genetic make-up is designated: phenotypic segregation. Heat resistance is first of all species specific (genetically determined) but the epigenetic segregation seems to be characteristic of the developmental process. This process can in certain mutants be affected by environmental conditions and more importantly by the so-called autoregulators (A-factor and factor C). Factor C and A-factor are needed to normal development, if their quantity or the time of addition to the culture was not optimal, the quantity of spores decreased.