Abstract
A total of 33 lactic acid bacteria (LAB) were isolated from 5 samples of traditional dairy product (Jben) collected from various livestock farms in Oued El Malha, Djelfa, the Ouled Naïl Range of north-central Algeria. The taxonomic study of the strain B04 using a multiphase approach based on morphology, physiology, molecular (16S rRNA) and phylogenetic analyses allowed correlating this strain to Enterococcaceae. The difference of the 16S rRNA gene sequence of the isolate toward the most closely related genus Enterococcus was more than 6 % suggesting that the strain B04 represents a new genus. The results of the evaluation of some physiological tests indicated that B04 exhibited good biological activities including acidifying, proteolytic and bacterial inhibition. The strain B04 was antagonistic toward Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, and Bacillus cereus ATCC 10876.
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