Phenotypic evaluation of interspecific recombinant inbred lines (RILs) of Phaseolus species for aluminium resistance and shoot and root growth response to aluminium–toxic acid soil

Abstract

Aluminium (Al) toxicity limits common bean productivity in acid soil regions of the tropics. To improve Al resistance of common bean, Al-sensitive Phaseolus vulgaris (SER16) was crossed to Al-resistant P. coccineus (G35346-3Q) to create 94 F5:6 recombinant inbred lines (RILs) of the pedigree SER16 × (SER16 × G35346-3Q). RILs were characterized for resistance to Al in a hydroponic system with 0 and 20 μM Al in solution, and for shoot and root growth response to Al-toxic infertile acid soil in 75 cm long soil cylinder system using an oxisol of low Al- (12.5%; pH 4.6; fertilized) and high Al-saturation (77%; pH 4.1; unfertilized). G35346-3Q increased its taproot elongation rate by 3.5% between 24 and 48 h under 20 μM Al in solution, while the best RIL, Andean genotype ICA Quimbaya, and sensitive genotype VAX1 expressed reductions of 2.6, 12.5, and 69.5%, respectively. In the acid soil treatment the correlation between leaf area and total root length was highly significant under high Al saturation (r = 0.70***). Genotypes that were Al resistant in the hydroponic system were not necessarily tolerant to Al-toxic acid soil conditions based on shoot and root growth responses. Phenotypic evaluation using both systems allows the identification of genotypes with Al resistance combined with acid soil adaptation. Two genotypes (ALB88 and ALB91) emerged as lines with multiple traits. Results suggest that inheritance of Al resistance and acid soil tolerance in G35346-3Q is complex. Results from this work will be useful for identification of molecular markers for Al resistance in Phaseolus species and to improve acid soil adaptation in common bean.