Phenotypic Characterization of Antimicrobial Resistance in Kerala Bacillus Spp Isolated from Paddy Field

Abstract

Rice paddy environment is an exceptional reservoir for different microorganisms (Pathogenic and Non-Pathogenic). Fertilizers, Fungicides, Antibiotics and Insecticides were used for boosting rice paddy growth and preventing agronomy diseases. Enormous use of fertilizers, Fungicides, Antibiotics and Insecticides will develop drug resistance in the microorganisms present in the crop [figure-1]. Ninety dried rice paddy samples were collected from different places around regions of Kerala. Forty rod-shaped gram-stain-Positive endospore forming obligate aerobic bacteria were isolated from the samples. All the forty strains were subjected to for antibiotic susceptibility by using the standard antibiotic disc (Cefoxitin). Significant drug resistance and massive growth observed in the strain (SCOP-2) among other isolated bacteria. SCOP-2 was subjected for sensitivity to differ different Antimicrobial disc by using Kirby-Bauer disc diffusion method. The results show that SCOP-2 drug resistant against all the five Antibiotics. The Minimum Inhibitory Concentration (MIC) was determined by two fold serial dilution against Ciprofloxacin (CIP) and Linezolid (LNZ). From the results it is concluded that CIP and LNZ shows resistance against SCOP-2.