Abstract
The sarcoma is the generic nomenclature for neoplasm of mesodermal cells, which express in man and animals. Silent growth requires early diagnosis technique for identifying their proteins. The experimental model in vivo murine sarcoma 180-TG (TG-180), is widely used in research to provide the stimuli of infectious and neoplastic antigens. In this case, the technique of immunohisto-chemistry helps identify the expressions of tumor cell variants. The objective of the research was to characterize immunoexpression murine sarcoma TG 180, by immunohistochemistry, antibodies AE1/AE3, vimentin, CD3, CD 45, CD79α and S100A4. For this, murine sarcoma TG-180, was implanted subcutaneously in 20 mice “Swiss”, male, 30 days old, 28 g for 10 days. Samples were taken and subjected to immunohistochemistry, via use of HistoMouse-MA™? kit. There was specifically labeled S100A4 and vimentin antibodies, indicative of poorly differentiated neoplasms fibroblasts. In fact, the model is established by identifying the origin of the cell, once identified, chemotherapeutic tests can also be performed. Neoplasia like these, when installed in man and animals, depending on the degree of aggressiveness requires treatment protocol varied between surgery and chemotherapy or combination of treatments.
Highlights
The use of animals in research has been an integral and indispensable part of the development of modern medicine, preventing massive human suffering [1]
Among the numerous currently existing experimental models, transplantable tumors of mice has been widely exploited in experimental oncology, mainly for biological and assessment of response to chemotherapy, since they are in low material cost and make the tracking of development in short time [2]-[5]
Beforehand, it is noteworthy that in the present study was consistent implantation and growth of the cells that make up the Murine Sarcoma TG-180, the dorsal region of the mice “Swiss” since shortly after the implant, which is 5 days already showed the turmoral development and the end of the experiment, 10 days after the inoculation, the lesions measured about 0.8 cm in diameter
Summary
The use of animals in research has been an integral and indispensable part of the development of modern medicine, preventing massive human suffering [1]. Among the numerous currently existing experimental models, transplantable tumors of mice has been widely exploited in experimental oncology, mainly for biological and assessment of response to chemotherapy, since they are in low material cost and make the tracking of development in short time [2]-[5]. In this line, tumors from different strains have been used for this purpose, including the murine sarcoma 180TG (TG-180). From transplantation studies, it was observed that their morphological characteristics and their behavior were suggestive of a mesenchymal tumor, and began to be called sarcoma 180 [6]
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