Phenotypic Characterization and bla Gene Detection in Antibiotic Resistant Escherichia coli Obtained from Poultry Fecal Samples

Abstract

Background: The current situation of multidrug resistant bacteria has become a worrisome issue in occurrence of disease outbreak. Multidrugresistant E. coli can be readily encountered in farms during daily clinical practice and veterinarian should act timely. Over past many reported increased incidences of Extended-Spectrum Beta-Lactamase (ESBL)-producing E. coli. The introduction of ESBL-producing E. coli from poultry farms to the environment may pose a health risk if these bacteria reach places where people may become exposed and we report that different methods of identifications. Methods: 60 fecal samples were collected from apparently healthy and infected poultry in the mixed age group. Bacterial isolates were identified on colony morphology and biochemical properties. ESBL producers were identified by using a combination disk diffusion method. ESBL positive isolates were further assessed using conventional polymerase chain reaction (PCR) to detect the TEM, SHV and CTX-M genes. Result: In the present study, 40 isolates of E.coli were isolated from 60 faecal samples. Out of 40 isolates, 12 isolates were resistant to two or more than two antibioticsand were positive for ESBL by combination disc test. CTX-M genes were detected in 6 (50%) isolates. TEM and SHV were not detected in any of the isolates. This study showed high resistance of E. coli to antibiotics, particularly in the third generation cephalosporins but were more susceptible to Gentamicin, Aztreonarm, Cefrtrazindime and Cefotoxime. Laboratory monitoring and detection of E. coli of ESBL producing bacteria important steps in the appropriate treatment for farm based poultry industry and infection control efforts.