Abstract
Non-small-cell lung cancer (NSCLC) is one of the main causes of death of malignant tumors of the respiratory system. At present, the clinical demand for biomarkers for predicting and diagnosing the disease is increasing. Overexpression of LncRNA Hotair (Homeobox transcriptional antisense intergenic RNA) has been previously reported to be associated with poor prognosis and high mortality in different malignancies. qRT-PCR results showed that the expression of LncRNA Hotair in tumor tissue and serum of patients with non-small-cell lung cancer was significantly upregulated. Clinicopathological correlation analysis showed that the upregulation of LncRNA Hotair expression was closely related to lymph node metastasis and tumor lymph node metastasis (TNM) stage (P < 0.05). The results showed that transfection of pcDNA3.1-Hotair could promote the expression of LncRNA Hotair in NSCLC, while transfection of Si-Hotair could reduce the expression level of LncRNA Hotair, hinder the migration and invasion of cancer cells, and promote cell apoptosis. After transfection of Si-Hotair, molecular markers related to migration, the level of E-cadherin and Bax, increased and the level of vimentin, Bcl-2, MMP-3, VEGF, Ki-67 and PCNA decreased. This shows that the proliferation and migration of A549 cells are promoted and LncRNA Hotair deletion can inhibit the proliferation and migration of lung cancer cells. These results show that the expression level of LncRNA Hotair of NSCLC cell lines can promote the invasion and migration of NSCLC, and its expression has a significant correlation with Lymph node metastasis, tumor size, and TNM stage. Therefore, this target is of great significance for the clinical diagnosis and treatment of NSCLC.
Highlights
Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors of the respiratory system. e main causes of death are smoking, low air quality, gene mutation, and other adverse factors, and its new cases in the world are increasing year by year, especially in China [1, 2]
Studies have shown that Long non-coding RNA (LncRNA) Hotair mainly interacts with Polycomb repressive complex 2 (PRC2) to regulate chromosome rearrangement and promote tumorigenesis[21]
Et al Found that LncRNA Hotair was highly expressed in liver cancer tissues and promoted hepatocarcinogenesis by inhibiting the expression of tumor suppressor genes; downregulating LncRNA Hotair expression can inhibit the growth and migration of hepatoma cells [22]
Summary
Non-small-cell lung cancer (NSCLC) is one of the most common malignant tumors of the respiratory system. e main causes of death are smoking, low air quality, gene mutation, and other adverse factors, and its new cases in the world are increasing year by year, especially in China [1, 2]. Plasma LncRNA-Pou3f3 can be used as a potential biomarker for the diagnosis of rectal cancer, especially early tumor screening [10]. LncRNA Loc389332 was found to be significantly increased in the plasma and urine of patients with renal clear cell carcinoma, which can be used to Journal of Healthcare Engineering distinguish cancer patients from the health examination group [11]. In oral squamous cell carcinoma, LncRNA Hotair overexpression enhances the metastatic potential and epithelial mesenchymal transformation characteristics of cancer cells. LncRNA Hotair can realize its function by reprogramming the chromosome state to control the expression of multiple genes represented by Hoxc; Hoxc has been proved to be a gene closely related to tumor pathogenesis, and its expression is significantly increased in cancer tissues of individuals with lymph node metastasis [16]
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