Abstract
The objective of this work was to evaluate if cryostorage of Phaseolus vulgaris L. seeds induced variations in regenerated plants at the phenotypic and molecular levels. A series of agricultural traits was measured on plants grown from control, non-cryopreserved and cryopreserved seeds, and the genetic stability of plants of the second generation was analysed at selected microsatellite loci. The phenotype of the second generation plants was evaluated as well. No statistically significant phenotypic differences were observed for the parameters measured, neither in the first nor in the second generations. Averaging both treatments, about 76% of the seeds had germinated 10 days after sowing. At harvest we recorded plants with about 73 cm in height, 13 stem internodes, 25 fruits, 103 grains and 4 grains per fruit. One hundred seeds weighted about 26 g. The genetic analyses performed on the second generation plants using six nuclear Simple Sequences Repeats (SSR) markers revealed no changes in microsatellite length between control and cryopreserved samples, implying that there was no effect of seed liquid nitrogen exposure on genome integrity. The phenotypic and molecular results reported here confirm that cryostorage is an efficient and reliable technique to conserve P. vulgaris seeds and regenerate true-to-type plants.
Highlights
Common bean (Phaseolus vulgaris L.) is one of the world’s most important grain legumes
The objective of this work was to evaluate if cryostorage of Phaseolus vulgaris L. seeds induced variations in regenerated plants at the phenotypic and molecular levels
The genetic analyses performed on the second generation plants using six nuclear Simple Sequences Repeats (SSR) markers revealed no changes in microsatellite length between control and cryopreserved samples, implying that there was no effect of seed liquid nitrogen exposure on genome integrity
Summary
Common bean (Phaseolus vulgaris L.) is one of the world’s most important grain legumes. It is consumed as dietary staple worldwide, especially in Latin America and Africa [1]. To improve the nutritional and agronomic characteristics of common bean varieties, numerous breeding programmes are carried out, which integrate conventional and advanced approaches. The Laboratory for Plant Breeding (University of Ciego de Avila, Cuba) is conducting a research project on P. vulgaris genetic improvement to increase tolerance to soil salinity. Cryopreservation finds relevant applications for long-term storage of non-orthodox seed species, and for that of orthodox seeds, as demonstrated by various research results [4]
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