Abstract
Recently, multiresistant Salmonella enterica serovar1,4,[5],12:i:-, a monophasic variant of S. Typhimurium(1,4,[5],12:i:1,2) emerged, and is now among the most common serovars isolated from humans in many countries.In Greece, monophasic Typhimurium which was recorded for the first time in human isolates in 2007(0.3% of total isolates), increased sharply thereafter,and since 2009 is the third most frequent serovar. In the present study, 119 S. enterica 1,4,[5],12:i:- strains of human, animal and food origin, isolated during the period between 2006 and 2011, were examined.Strains verified as monophasic Typhimurium variants by polymerase chain reaction (PCR) (97 strains), were further characterised by phenotypic (antibiotic resistance and phage typing) and molecular (pulsed-field gel electrophoresis – PFGE) methods. The results indicate that multiple clones of multiresistant monophasic Typhimurium are circulating in Greece. The most frequently encountered clone in humans and pigs was that of phage type DT120, R-type ASSuTSpTm and PFGEprofile STYMXB.0010, while in poultry other clones were detected. The data indicate that pigs may be a reservoir of this clone in Greece.
Highlights
Salmonella enterica serovars Enteritidis and Typhimurium have been reported to be the most common causes of human salmonellosis worldwide [1,2]
The number of cases infected with multiresistant S. enterica serovar 1,4,[5],12:i:- increased and, it is difficult to monitor trends of monophasic strains because of the inconsistent way reported by different countries and organisations internationally, it is considered to be among the ten most common serovars isolated from humans in many countries in Europe [5] and the United States of America (US) [2]
We present the results of the phenotypic and molecular characterisation of S. serovar 1,4,[5],12:i:- strains, of human, food and animal origin isolated in Greece since 2006, with respect to their antimicrobial susceptibility profile, phage type and DNA fingerprinting using the pulsed-field gel electrophoresis (PFGE) method
Summary
Salmonella enterica serovars Enteritidis and Typhimurium have been reported to be the most common causes of human salmonellosis worldwide [1,2]. The number of cases infected with multiresistant S. enterica serovar 1,4,[5],12:i:- increased and, it is difficult to monitor trends of monophasic strains because of the inconsistent way reported by different countries and organisations internationally, it is considered to be among the ten most common serovars isolated from humans in many countries in Europe [5] and the United States of America (US) [2]. Typhimurium monophasic variants, and to discriminate from the other serovars that share the same somatic and flagellar (phase 1) antigens [5]. It is not as yet clear if infections caused by S. Typhimurium and S. enterica serovar 1,4,[5],12:i:strains differ in severity, even though studies so far indicate that both serovars present similar virulence mechanisms [6,7,8,9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
