Phenotypic and genotypic study associated with biofilm formation for E. coli isolated from UPEC

Abstract

UTI is a serious health problem with respect to antibiotic resistance and biofilms formation being the prime cause for the antibiotic resistance. In current study sixty E.coli isolated collected from patients with urinary tract infection. In this study, biofilm formation were detect by phenotypic and genotypic methods. The detection of biofilm formation by phenotypic method was carried out by Congo Red Agar (CRA) method and tissue culture plate (TCP) method and the genotypic method performed by detection present some gene associated with biofilm formation which include fliC ,csgA ,fimH and papC.. The results showed that 54(90%) from E. coli produced strong and moderate slime layer. The present study showed that 57(95%) from E. coli produced strong and moderate biofilm. Genotypic study of E. coli biofilm-associated genes were found in various frequencies in all isolates that included fliC (63.3%), csgA (73.3%), fimH (90%) and papC (76.6%).