Abstract
BackgroundCystic fibrosis (CF) lung microbiota consists of diverse species which are pathogens or opportunists or have unknown pathogenicity. Here we report the full characterization of a recently described multidrug resistant bacterium, Microbacterium yannicii, isolated from a CF patient who previously underwent lung transplantation.ResultsOur strain PS01 (CSUR-P191) is an aerobic, rod shaped, non-motile, yellow pigmented, gram positive, oxidase negative and catalase positive bacterial isolate. Full length 16S rRNA gene sequence showed 98.8% similarity with Microbacterium yannicii G72T type strain, which was previously isolated from Arabidopsis thaliana. The genome size is 3.95Mb, with an average G+C content of 69.5%. In silico DNA-DNA hybridization analysis between our Microbacterium yannicii PS01isolate in comparison with Microbacterium testaceum StLB037 and Microbacterium laevaniformans OR221 genomes revealed very weak relationship with only 28% and 25% genome coverage, respectively. Our strain, as compared to the type strain, was resistant to erythromycin because of the presence of a new erm 43 gene encoding a 23S rRNA N-6-methyltransferase in its genome which was not detected in the reference strain. Interestingly, our patient received azithromycin 250 mg daily for bronchiolitis obliterans syndrome for more than one year before the isolation of this bacterium.ConclusionsAlthough significance of isolating this bacterium remains uncertain in terms of clinical evolution, this bacterium could be considered as an opportunistic human pathogen as previously reported for other species in this genus, especially in immunocompromised patients.
Highlights
Cystic fibrosis (CF) lung microbiota consists of diverse species which are pathogens or opportunists or have unknown pathogenicity
Some of the biochemical tests for our strain yielded results similar to those reported for M. yannicii G72 type strain [14], we found at least nine differences between our isolate and the type strain that are presented in Table 1 along with comparison to the three other type strains
Our results showed that CF Microbacterium yannicii, which has previously been isolated from Arabidopsis thaliana roots, has never been reported from a human clinical specimen and its pathogenicity in this context is unknown
Summary
Cystic fibrosis (CF) lung microbiota consists of diverse species which are pathogens or opportunists or have unknown pathogenicity. We report the full characterization of a recently described multidrug resistant bacterium, Microbacterium yannicii, isolated from a CF patient who previously underwent lung transplantation. Many new, emerging and/or multidrug resistant bacteria have been recently reported in CF patients using different technologies including new culture media and molecular methods [3,8,11,12]. We report the isolation and full description of Microbacterium yannicii isolated from the sputum sample from a lung transplanted CF adult patient for which we have recently published the genome sequence [13]. To the best of our knowledge, bacteria of this genus have never been reported in clinical samples from CF patients. We present a full description of phenotypic and genomic properties of this new bacterium isolated from a CF sputum sample
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