Abstract
Back ground: Microcins are ribosomal synthesized antimicrobial peptides produced principally by bacteria of the Enterobacteriaceae family that are active against other bacteria, either in the same species (narrow spectrum), or across genera (broad spectrum) that share the same ecological niche.
 Objective: The ability of the Enterobacteriaceae to produce microsines.
 Materials and methods: One hundred urine samples have been collected from patients suffering from UTI whom admitted to Al Hakim Hospital in AL-Najaf City during the period from 17October 2017 to 13 February 2018.
 Results: The results of PCR technique for amplification show that all isolates were possess mic N by appearance of amplicon with molecular weight 938 bp while 46 (76%) isolates were possess mic N1and 24 (40%) isolates were possess mic N2 gene by appearance of amplicon with molecular weight 368 bp and 167 bp respectively.Cross-streak activity assay was used to detect the selected strains with antibacterial activities against pathogenic bacteria.
 Conclusion: some isolates have one or more gene microcin encoded of , however, microcin N is present in all isolates.
Highlights
The family Enterobacteriaceae comprises a large heterogeneous group of Gram-negative, they are uncommonly found outside the gastrointestinal tract, they are a leading cause of nosocomial disease [1,2]
Bacteria engender antimicrobial compounds to inhibit or kill other competing strains because they survive in the environment and their competition with other microorganisms for purse.[4] .This antimicrobial peptides are just one of the many classes of antimicrobial component, One of these peptides, microcin N are low-molecular-weight (10 000 Da) antimicrobial peptides secreted by members of the Enterobacteriaceae family and are associated in microbial competition inside the intestinal tract
The result of isolation and identification of Enterobacteriaceae members on CHROM Agar showed a wide distribution of Enterobacteriaceae members among all collected sample in which all 100 (100%) sample gave +ve culture
Summary
The results of PCR technique for amplification show that all isolates were possess mic N by appearance of amplicon with molecular weight 938 bp while 46 (76%) isolates were possess mic N1and 24 (40%) isolates were possess mic N2 gene by appearance of amplicon with molecular weight 368 bp and 167 bp respectively.Cross-streak activity assay was used to detect the selected strains with antibacterial activities against pathogenic bacteria.
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