Abstract
A new Bacillus cereus phage, SWEP1, was isolated from black soil. The host lysis activity of phage SWEP1 has a relatively short latent time (20min) and a small burst size of 83 PFU. The genome of SWEP1 consists of 162,461bp with 37.77% G+C content. The phage encodes 278 predicted proteins, 103 of which were assigned functionally. No tRNA genes were found. Comparative genomics analysis indicated that SWEP1 is related to Bacillus phage B4 (86.91% identity, 90% query coverage). Phenotypic and genotypic characterization suggested that SWEP1 is a new member of a new species in the genus Bequatrovirus, family Herelleviridae.
Highlights
Bacillus cereus, a ubiquitous bacterium in the environment, such as soil, water, air, food, and animal intestines [1]
The general features of some B. cereus bacteriophages are listed in Supplementary Table 1
The phages of B. cereus that have been isolated were extracted from soil [4,5,6,7], mud [8, 9], fermented foods [10, 11], lakes [12], and animal carcasses [13]
Summary
A ubiquitous bacterium in the environment, such as soil, water, air, food, and animal intestines [1]. The whole genome of phage SWEP1 was completely sequenced and analyzed. The host bacterium, designated strain Bacillus cereus LB2 (=CGMCC 1.18775), was isolated on LB agar medium [18] and taxonomically assigned based on the whole genome comparison.
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