Abstract
Background: The Extended spectrum β-lactamases (ESBLs) production is one of the most essential mechanism of drug resistance in Proteus mirabilis (P. mirabilis). Objective: Phenotypic and genotypic detection of extended spectrum β -lactamases production in P. mirabilis and its antimicrobial resistance among clinical isolates. Methods: A total of Sixty P. mirabilis clinical isolates were tested for Extended spectrum β lactamase production by modified double disc synergy test (MDDST). Polymerase chain reaction (PCR) was done to detect Extended spectrum β-lactamases genes in the clinical isolates. Antimicrobial susceptibility testing was done by means of Kirby Bauer disc diffusion method. Results: Out of the 60 P. mirabilis clinical isolates, 17 isolates (28.33%) were ESBL producers by phenotypic test. Genotypically; ESBLs genes were detected and the most prevalent ESBL resistance gene was TEM (91.7%) followed by SHV (75%), CTX-2-59 (56.7%) and CtxM-15 (51.7%). Moderate level of resistance to ofloxacin, ciprofloxacin, amoxicillin/ clavulanic acid, cefotaxime and ceftazidime (60.0%, 58.3%, 45.0%, 41.7% and 40.0% respectively) was recorded. In addition, 73.33% of isolates were classified as multidrug resistant (MDR). Conclusion: Monitoring of Extended spectrum β-lactamase producing P. mirabilis is very important because of its high prevalence among urinary tract infections. Also; increasing awareness for clinicians and enhancing laboratories
Highlights
Proteus mirabilis (P. mirabilis) is a Gram-negative rod-shaped bacterium frequently noted for its swarming motility and urease activity
Failure of treatment and clinical morbidity were extra predictable to happen in patients infected by ESBLs-producing P. mirabilis, which had been estimated to inadequate therapy (Wang et al, 2014)
World Health Organization (WHO) reported that the most serious problem was the routine use of antibiotics leading to the incidence of antimicrobial drug resistant and development of antimicrobial resistant genes
Summary
Proteus mirabilis (P. mirabilis) is a Gram-negative rod-shaped bacterium frequently noted for its swarming motility and urease activity It considers as an important uropathogen between patients with complicated urinary tract, urolithiasis and extended-term urinary catheterization (CAUTI). Current study aimed to explore the genotype of ESBLs of P. mirabilis clinical isolates isolated from urinary tract infection from Urology and Nephrology center, Mansoura University Hospitals, Dakahlia governorate, Egypt, with recognition of their antibiotic resistance. The Extended spectrum β-lactamases (ESBLs) production is one of the most essential mechanism of drug resistance in Proteus mirabilis (P. mirabilis). Objective: Phenotypic and genotypic detection of extended spectrum β -lactamases production in P. mirabilis and its antimicrobial resistance among clinical isolates. Conclusion: Monitoring of Extended spectrum β-lactamase producing P. mirabilis is very important because of its high prevalence among urinary tract infections. ; increasing awareness for clinicians and enhancing laboratories tests leading to reduce the spread of these isolates
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