Abstract
Phenoloxidase has been localized in the epicuticle, exocuticle, and epidermal cells of the mole crab, Emerita asiatica Milne Edwards. The enzyme activity in different moulting stages is in the order of freshmoult > premoult > intermoult = postmoult. The phenoloxidase of the freshmoult cuticle oxidizes pyrogallol and epinephrine (adrenaline) more effectively than the other phenols studied. There is no monophenolase activity. The possible metabolic pathway has been suggested based on the specificity of the enzyme and the chromatographic identification of the extracted phenols. Phenoloxidase shows different pH optima in different buffers. The protein pattern in the various moulting stages of the cuticle differs and the results are discussed in relation to sclerotization.
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