Phenolic substances as regulators of the intensity of lipid peroxidation processes of the strains Pleurotus eryngii, Fistulina hepatica and Agrocybe cylindracea

O V Fedotov

doi:10.15421/022034

Abstract

The work is devoted to the calculation, comparison of indicators and the development of a method for regulating the intensity of lipid peroxidation processes (LPP) of strains of basidiomycetes. The purpose of the investigation is to study the effect of phenolic type chemicals and hydrogen peroxide on the lipid peroxidation of certain strains of basidiomycetes under laboratory cultivation. Cultivation of strains of basidiomycetes was carried out by periodic surface method on glucose-peptone medium (GPM) in flasks. The influence of sodium lignosulfonate, tannin, gallic acid and hydrogen peroxide at 0.1% concentration at 24 and 48 hours of exposure on the intensity of lipid peroxidation processes of the strains Pleurotus eryngii P-er, Fistulina hepatica Fh-08 and Agrocybe cylindracea 960, fungi of the phylum Basidiomycota, orderAgaricales has been investigated. It was established that the used phenolic-type chemical compounds that are part of the lignocellulose complex of wood or are the products of its decomposition to a certain extent affect the lipid peroxidation processes of mycelial cell lipids in the studied cultures of basidium fungi. The individual reaction of LPP of cultures to the applied substance and the time of its exposure are determined. The highest degree of LPP induction was recorded upon addition of tannin – by 161%, after 48 hours of exposure in the mycelium of strain Fh-08; sodium lignosulfonate – by 192%, after 48 hours in the mycelium of strain P-er; gallic acid – by 182%, after 24 hours, in the mycelium of strain P-er; hydrogen peroxide – by 257%, after 24 hours, in the CR of strain 960. The biotechnological significance of this is the possibility of regulation (induction or repression) of LPP of producer strains. From a biomedical point of view, the possibility of changing the activity of lipid peroxidation processes of mycelium and culture fluid makes it possible to obtain and use more effective functional products of fungal origin.

Highlights

Reactive oxygen species (ROS) are constantly formed in a living cell as products of normal oxygen metabolism
It was found that the spontaneous intensity of the LPP (LPPs) processes of the mycelium of all strains was significantly higher than this indicator in the culture fluid
The intensity of lipid peroxidation of mycelium increased with the age of the culture, which can be explained by a growing shortage of certain nutrients and an increase in the number and concentration of metabolic products in the culture fluid

Summary

Introduction

Reactive oxygen species (ROS) are constantly formed in a living cell as products of normal oxygen metabolism. Various environmental factors, including teratogenic factors, have been identified in this process. The study of this effect is of practical interest, since ROS can play the role of mediators of important intracellular signaling pathways, inducers of the defense system and ion transport systems, trigger programmed cell apoptosis, etc. Increased of ROS production leads to the development of oxidative stress and is a serious problem for a living organism. The antioxidant system counteracts the ROS and maintains the oxidation-reduction balance of the status of the organism (Bennett et al, 2017; Obrador et al, 2019)

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