Abstract

Although Australia is the largest exporter of faba bean globally, there is limited information available on the levels of bioactive compounds found in current commercial faba bean varieties grown in this country. This study profiled the phenolic acid and flavonoid composition of 10 Australian faba bean varieties, grown at two different locations. Phenolic profiling by HPLC-DAD revealed the most abundant flavonoid to be catechin, followed by rutin. For the phenolic acids, syringic acid was found in high concentrations (72.4–122.5 mg/kg), while protocatechuic, vanillic, p-hydroxybenzoic, chlorogenic, p-coumaric, and trans-ferulic acid were all found in low concentrations. The content of most individual phenolics varied significantly with the variety, while some effect of the growing location was also observed. This information could be used by food processors and plant breeders to maximise the potential health benefits of Australian-grown faba bean.

Highlights

  • Faba bean (Vicia faba L.) is reported to be the third most important legume crop [1], with over 5.4 million tonnes being harvested globally in 2019 [1]

  • Baginsky et al [13] found clear differences in the phenolic composition of 10 faba bean varieties grown in Chile, it should be noted that this study was performed on immature seed material

  • A total of 10 phenolic compounds were identified in the faba bean extracts (Figure 2), comprising of four hydroxybenzoic acids, three hydroxycinnamic acids and three flavonoidrelated compounds (Table 1)

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Summary

Introduction

Faba bean (Vicia faba L.) is reported to be the third most important legume crop [1], with over 5.4 million tonnes being harvested globally in 2019 [1]. Reported health benefits include improving cardiovascular health [5], providing anti-obesity effects [5,6], anti-cancer activity [5], anti-inflammatory activity [7] and inhibiting xanthine oxidase [8]. Baginsky et al [13] found clear differences in the phenolic composition of 10 faba bean varieties grown in Chile, it should be noted that this study was performed on immature seed material Another earlier study highlighted the range in total phenolic contents and antioxidant activity among 13 Tunisian faba bean cultivars [14]. NNasaasra-rA-Abbbbaasseettaall.. [[1155]] rreeppoorrtteedd oonntthheepphheennoolilciscsfofuonudndininonoenAe uAsutrsatlriaanliafanbfaabbeaabnean vavraiertieyt,yw, whhilieleSSiaiahh eett aall..[1[166] ]anadndSiaShiaeht aelt. [a5l].i[n5v]eisntivgeastteidgatwteodatnwdotharneedvtahrrieeteievsa, rreiestpieecs-, reAsaripceutitatteatiooilsvivcnttetahteairsitiflolavd,yyaaul,ae.binigtallSnaotythnihh.tabfaiooihfelSnlcaluaieidaybnngatniaaghhavnvtlihtb.iaidehnn[oertu1iidcasce7aaynoitk]llvian.enspvintts[doheua1i.wunnerd7Rnita]licee,eeolocsatddlpietnniegucnhttdsehedtea.lnetgncyioRetiap,otd,depahJlasoicl,ecntehoppnhdannrhroecetsetlflisioolpnydcaent,ohvsnacJleooteloioitcnnnpnrhaootchgflneloil.endipasnn[ctovsh1toeanc8oewlnno]nniectednorotroecltiaeipefadocnnlon1nst.p0rtoitw[rotet1aAeoxnedi8nfinutird]dlevosoeartensfennrasa1pontottl0hinaotitgaiecrAiotantnthatixeuvveefnidasdiedt1ttbis.yrao0oatTannixiflgbnhaitideaatibfsahatnaiavecnenwdtfbetiavaoe.vAbaanarTicakrnuttthiyiiseboavvittesiixraiimneatariwydslnis--fi,,oavvnraketaimetisetsostauiddiiendfiblylinJoghtnhsiosnknetoawl.l[e1d8g].eIgt aisph, oppreesdetnhtaint gthpishwenilollpicropvroidfielefsurotnhetrhien1si0gfhatbiantboean vatrhieetineustsrittuiodnieadl abnydJohhenalstohn-beetnaelf.it[t1in8g]. pItriospheortpieesdotfhcaotmthmisowniAll upsrtorvaliidaenffuarbtahebreiannsicguhltti-into thveanrus,traistiwonealllaasnpdrohveiadltinhg-bveanleufiatbtlienginpforrompeartitoiensoonf cthoememxtoenntAouf sthtreairliagnenfoatbyapbiceavnarciautlitoinvars, aspwreeslel natsipnrtoevrmidsinogf pvahleunaoblilce aincifdoramndatfiloanvoonnotihdebeioxsteynntthoefstihsepiartghewnaoytys.pic variation present in terms of phenolic acid and flavonoid biosynthesis pathways

Results and Discussion
Phenolic acid Profiling by HPLC
Principal Component Analysis and Correlation Analysis
Extraction of Phenolic Compounds
Phenolic Profiling by HPLC
Data Analysis
Conclusions
Full Text
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